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The software almost has too many features to comment on in any one review (e.g.
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Based on the hours of time this program has saved me already I would not even blink at the academic price tag of $133 (USD) - or cheaper for students.
GENEIOUS PRIME PRICE PRO
The Geneious team has made available a freeware version and a pro version that (for a price) allows you to access more functionality. Moreover, the software is structured in such a way that “new” plugins can be written and incorporated with relative ease. My request to provide a variety of nucleotide colour schemes was implemented within 2 weeks. As is the case with any new software there are a few features that don't work quite as well as you might like - but the good news is that if you write to them they will likely fix it up asap. With the integration of sequence chromatograph viewing and editing of DNA sequences/alignments (new in version 2) Geneious is now firmly embedded in my research practices. If you are looking for a piece of bioinformatics software for teaching purposes look no further than Geneious. Students did a Genbank search, downloaded files, did an alignment and built a phylogenetic tree (with bootstrapping) all in the blink of an eye. Earlier this year I used the Beta-release in some undergraduate teaching labs - with great success.
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While the software has some “bell and whistles” for more complex tasks one of its key attributes is a simple user interface. The user interface of Geneious is both attractive and intuitive. The software logo “research in a flash” accurately describe the potential of Geneious - only months after its release it has become the “Swiss army knife” of bioinformatics software. Current and future releases of Geneious (offer a solution to (most) of these frustrations. Chopping and changing between different file formats and at times different operating systems (Mac, PC, Unix/Linux) has been a frustrating and time-consuming exercise. We show that our method is not limited to honey but may also be applied to other honeybee products, such as royal jelly.For a long time scientists who generate or analyse DNA sequences have been using multiple pieces of software to: 1) view chromatographs, 2) trim sequences 3) Blast data 4) align sequences 5) build phylogenetic trees. Additionally, EPIC markers are also useful in detecting heterozygosity, for example, in tracing paternal and maternal lines in contrast to abundantly used mitochondrial tRNA Leu – cox2, which is inherited via maternal line only and may thus significantly contribute to the resolving of paternal relationships. Our results demonstrate the correct assignment of the samples to their entomological origin on the interspecific level.įurthermore, PCR amplification using the designed EPIC marker and double restriction protocol was demonstrated as useful in detecting mixed samples as well as two mislabelled honey samples claimed to belong to Asian honeybee Apis cerana.
GENEIOUS PRIME PRICE VERIFICATION
Verification of the approach was performed with 25 tissue samples and tested additionally with 20 honey samples and 1 royal jelly sample. Amplified fragments were differentially double digested by restriction endonucleases MnlI and HpaI, which resulted in specific restriction patterns observed on agarose gel electrophoresis. Based on successful sequencing and phylogenetic analyses, we identified a short interspecifically variable part in the ANT gene for which we designed PCR primers that allow the successful amplification of fragmented DNA from sources like honey. For confirmation of the samples’ identity, we used widespread mitochondrial tRNALeu – cox2 marker. Initially, we constructed EPIC markers for a nuclear single-copy adenine nucleotide translocase (ANT) gene to explore the properties of interspecific variation in honeybee tissue samples. Here, we report on new DNA-based tools for the correct identification of the DNA signatures of three economically important honeybee species: Apis mellifera, Apis cerana, and Apis dorsata. The entomological origin may be determined through DNA-based techniques. Such types of honey are often subject to various fraudulent practices, such as mislabelling of the origin or admixing with lower-cost and low-quality honey which does not match the label declaration. For example, honey from native and/or non-domesticated species, such as Asian Apis dorsata and Apis cerana, is more valued and achieves higher prices compared to the honey from managed colonies of A. The entomological source of honey is linked to its origin and may substantially contribute to the end products' price.